Extended Data Fig. 4: The expression and activity of Myc897-Piezo1 in the LHA.
From: In vivo magnetogenetics for cell-type-specific targeting and modulation of brain circuits
(a) Immunohistochemistry images of brain slices showing Piezo1 expression in the LHA region of Vglut2-Piezo1 mouse. Blue, DAPI (nucleus); Green, Piezo1. Scale bar, 200 μm. (b) Membrane expression of Piezo1 in the LHA region of Vgat-Piezo1 mouse. Blue, DAPI (nucleus); Green, Piezo1. Scale bar, 20 μm. (c, d) Fluorescence histology images of brain slices showing Piezo1 (green) and c-Fos expression (red) at the LHA of Vgat- or Vglut2-Piezo1 mouse upon Yoda1 treatment. Blue, DAPI (nucleus). The expression of c-Fos was detected only in the Piezo1-expressing mice upon Yoda1 treatment, confirming the function of Piezo1 ion channels. Scale bars, 50 μm.