Extended Data Fig. 3: Electrophysiological recording of Piezo1-expressing HEK cells in response to MMG stimulation.
From: In vivo magnetogenetics for cell-type-specific targeting and modulation of brain circuits
(a) Confocal microscope images of a Piezo1-expressing HEK293 cell labeled with 200 nm m-Torquer on the cell surface. (b) Schematic illustration showing a patch-clamp set-up for electrophysiological recording during MMG stimulation. (c) Whole-cell voltage-clamp trace (at −60 mV) from a Piezo1-expressing (black) and an EGFP-transfected (grey) HEK293 cell evoked by a single pulse (180° s-1) MMG stimulation. (right inset) Quantification of peak current amplitudes. Data are the mean ± s.d. of n = 11 for Vehicle; n = 10 for Piezo1 biological independent samples. ***p = 0.0003; two-tailed unpaired Student’s t-test with Welch’s correction. (d) Current traces recorded from a Piezo1- and EGFP-expressing HEK cell in response to a train of 6 repetitive magnetic pulses. Each black bar represents a single magnetic pulse with a 1.0 s duration at 180° s-1 rotating speed.