Extended Data Fig. 2: In situ calcium influx analysis of neurons with X-Rhod-1.
From: In vivo magnetogenetics for cell-type-specific targeting and modulation of brain circuits
(a) False-colour coded images of fluorescence of neurons with and without Piezo1 expression, m-Torquer, or rotating magnetic field, respectively. A single magnetic pulse was given at t = 0 s for 0.5 s. (b) Calcium fluorescence intensity plots corresponding to images in (a). Rapid increase of calcium influx was observed only for the m-Torquer-bound Piezo1-expressing neurons in response to magnetic stimulation, while all control neurons without either m-Torquer, Piezo1 expression, or magnetic field showed no calcium response.