Extended Data Fig. 5: Histological analysis against in vivo c-Fos expression in the LHA of Vgat- or Vglut2-Cre mice.
From: In vivo magnetogenetics for cell-type-specific targeting and modulation of brain circuits
(a, b) Fluorescence histology images against c-Fos (red) to evaluate in vivo neuronal excitation in LHA of (a) Vgat-Cre or (b) Vglut2-Cre mice under various conditions. Extensive MMG-driven c-Fos expression was observed only in the presence of both m-Torquer and Piezo1 with magnetic stimulation. Blue, DAPI (nucleus). (c, d) Quantification of c-Fos-positive cells in the LHA of (c) Vgat-Cre or (d) Vglut2-Cre under the eight conditions. (a, b) Scale bars, 50 µm. (c, d) One-way ANOVA with multiple comparison test; F(7, 16) = 41.42, p < 0.0001 for (c). Data are mean ± s.d. **p = 0.0027; n.s., non-significant; two-tailed unpaired Student’s t-test for (c). One-way ANOVA with multiple comparison test; F(7, 16) = 81.95, p < 0.0001 for (d). Data are mean ± s.d. ***p = 0.0008; n.s., non-significant; two-tailed unpaired Student’s t-test for (d). All data shown are the mean ± s.d. of n = 3 animals.