NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE79306 Query DataSets for GSE79306
Status Public on Jul 12, 2017
Title Gene expression profiles of brain endothelial cells during embryonic development at bulk and single-cell levels
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Other
Summary Purpose: Exploring mechanisms defining the unique nature of vascular development and differentiation in the brain .
Methods: High-resolution gene expression profiles of embryonic endothelial cells (EC) using translating ribosome affinity purification (TRAP) and single cell RNA-sequencing. RNA-sequenzing of transcription factor infected HUVEC.
Results: Comparisons of different organ-, temporal- or Ctnnb1 knock out-specific vascular translatomes revealed extensive molecular changes during the unique development of the brain endothelium. We identified brain endothelial-specific transcription factors – Foxf2, Foxl2, Foxq1, Lef1, Ppard, Zfp551 and Zic3 – that are associated with the temporal maturation of the blood-brain barrier and act downstream of the Wnt/Ctnnb1 signaling pathway. Profiling individual EC revealed a remarkable heterogeneity. Nevertheless, high levels of Foxf2, Foxq1, Ppard and Zic3 were correlated with elevated expression of differentiation markers. This could be recapitulated in vitro, where expression of Foxf2 and Zic3 in HUVEC led to an induction of blood-brain barrier differentiation markers.
Conclusions: Our study provides further insights into the temporal and cellular complexity of the developing embryonic brain endothelium. Our data raises many questions and provides a basis for further studies aiming to unravel cellular and molecular mechanisms that underlie vascular development and differentiation in the CNS. We anticipate that additional investigation of the identified transcription factors is likely to be a fruitful approach in understanding and manipulating the development of BBB and to engineer more advanced blood brain barrier in vitro models.
 
Overall design TRAP-seq profiles for E11.5 till E17.5 wild type (WT) brain EC; E14.5 and E17.5 endothelial Ctnnb1 -/- brain EC; E14.5 head, heart, kidney, limb, liver and lung WT EC in triplicate, using Illumina HiSeq 2000. Single cell RNA-sequencing of E14.5 brain EC. RNA-sequenzing of HUVEC infected with either control, Foxf2, Foxq1, ZIC3 or three transcription factors combined in triplicate.
 
Contributor(s) Hupe M, Li MX, Kneitz S, Davydova D, Yokota C, Kele-Olovsson J, Hot B, Gessler M, Stenman JM
Citation(s) 28698213
Submission date Mar 16, 2016
Last update date May 15, 2019
Contact name Mike Hupe
E-mail(s) Mike.Hupe@uni-wuerzburg.de
Phone +499313184906
Organization name University of Würzburg
Department Department of Developmental Biochemistry
Street address Biocenter Am Hubland
City Würzburg
ZIP/Postal code D-97074
Country Germany
 
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (175)
GSM2091161 Brain_E115_EC_Translatome_1
GSM2091162 Brain_E115_EC_Translatome_2
GSM2091163 Brain_E115_EC_Translatome_3
Relations
BioProject PRJNA315441
SRA SRP071876

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE79306_Deseq_Foxf2+Foxq1+ZIC3_vs_Control.txt.gz 1.4 Mb (ftp)(http) TXT
GSE79306_Deseq_Foxf2_vs_Control.txt.gz 1.4 Mb (ftp)(http) TXT
GSE79306_Deseq_Foxq1_vs_Control.txt.gz 1.4 Mb (ftp)(http) TXT
GSE79306_Deseq_ZIC3_vs_Control.txt.gz 1.4 Mb (ftp)(http) TXT
GSE79306_RPKM_SingleCell.txt.gz 4.4 Mb (ftp)(http) TXT
GSE79306_deseq_E115_brain_EC_vs_E115_brain_total.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E125_brain_EC_vs_E115_brain_EC.txt.gz 1.9 Mb (ftp)(http) TXT
GSE79306_deseq_E125_brain_EC_vs_E125_brain_total.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E135_brain_EC_vs_E115_brain_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E135_brain_EC_vs_E125_brain_EC.txt.gz 1.9 Mb (ftp)(http) TXT
GSE79306_deseq_E135_brain_EC_vs_E135_brain_total.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_KO_brain_EC_vs_E145_KO_brain_total.txt.gz 2.1 Mb (ftp)(http) TXT
GSE79306_deseq_E145_brain_EC_vs_E115_brain_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_brain_EC_vs_E125_brain_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_brain_EC_vs_E135_brain_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_head_EC_vs_E145_brain_EC.txt.gz 2.1 Mb (ftp)(http) TXT
GSE79306_deseq_E145_head_EC_vs_E145_head_total.txt.gz 2.2 Mb (ftp)(http) TXT
GSE79306_deseq_E145_head_EC_vs_E145_heart_EC.txt.gz 2.1 Mb (ftp)(http) TXT
GSE79306_deseq_E145_head_EC_vs_E145_kidney_EC.txt.gz 2.1 Mb (ftp)(http) TXT
GSE79306_deseq_E145_head_EC_vs_E145_limb_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_head_EC_vs_E145_liver_EC.txt.gz 2.1 Mb (ftp)(http) TXT
GSE79306_deseq_E145_head_EC_vs_E145_lung_EC.txt.gz 2.1 Mb (ftp)(http) TXT
GSE79306_deseq_E145_heart_EC_vs_E145_brain_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_heart_EC_vs_E145_head_EC.txt.gz 2.1 Mb (ftp)(http) TXT
GSE79306_deseq_E145_heart_EC_vs_E145_heart_total.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_heart_EC_vs_E145_kidney_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_heart_EC_vs_E145_limb_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_heart_EC_vs_E145_liver_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_heart_EC_vs_E145_lung_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_limb_EC_vs_E145_brain_EC.txt.gz 2.1 Mb (ftp)(http) TXT
GSE79306_deseq_E145_limb_EC_vs_E145_head_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_limb_EC_vs_E145_heart_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_limb_EC_vs_E145_kidney_EC.txt.gz 2.1 Mb (ftp)(http) TXT
GSE79306_deseq_E145_limb_EC_vs_E145_limb_total.txt.gz 2.1 Mb (ftp)(http) TXT
GSE79306_deseq_E145_limb_EC_vs_E145_liver_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_limb_EC_vs_E145_lung_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_liver_EC_vs_E145_brain_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_liver_EC_vs_E145_head_EC.txt.gz 2.1 Mb (ftp)(http) TXT
GSE79306_deseq_E145_liver_EC_vs_E145_heart_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_liver_EC_vs_E145_kidney_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_liver_EC_vs_E145_limb_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_liver_EC_vs_E145_liver_total.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_liver_EC_vs_E145_lung_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_lung_EC_vs_E145_brain_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_lung_EC_vs_E145_head_EC.txt.gz 2.1 Mb (ftp)(http) TXT
GSE79306_deseq_E145_lung_EC_vs_E145_heart_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_lung_EC_vs_E145_kidney_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_lung_EC_vs_E145_limb_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_lung_EC_vs_E145_liver_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_lung_EC_vs_E145_lung_total.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_wt_brain_EC_vs_E145_KO_brain_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E145_wt_brain_total_vs_E145_KO_brain_total.txt.gz 2.1 Mb (ftp)(http) TXT
GSE79306_deseq_E155_brain_EC_vs_E115_brain_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E155_brain_EC_vs_E145_brain_EC.txt.gz 886.2 Kb (ftp)(http) TXT
GSE79306_deseq_E155_brain_EC_vs_E155_brain_total.txt.gz 2.1 Mb (ftp)(http) TXT
GSE79306_deseq_E165_brain_EC_vs_E115_brain_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E165_brain_EC_vs_E155_brain_EC.txt.gz 851.1 Kb (ftp)(http) TXT
GSE79306_deseq_E165_brain_EC_vs_E165_brain_total.txt.gz 2.1 Mb (ftp)(http) TXT
GSE79306_deseq_E175_KO_brain_EC_vs_E175_KO_brain_total.txt.gz 2.3 Mb (ftp)(http) TXT
GSE79306_deseq_E175_brain_EC_vs_E115_brain_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E175_brain_EC_vs_E125_brain_EC.txt.gz 980.8 Kb (ftp)(http) TXT
GSE79306_deseq_E175_brain_EC_vs_E135_brain_EC.txt.gz 983.2 Kb (ftp)(http) TXT
GSE79306_deseq_E175_brain_EC_vs_E145_brain_EC.txt.gz 2.0 Mb (ftp)(http) TXT
GSE79306_deseq_E175_brain_EC_vs_E155_brain_EC.txt.gz 991.9 Kb (ftp)(http) TXT
GSE79306_deseq_E175_brain_EC_vs_E165_brain_EC.txt.gz 875.0 Kb (ftp)(http) TXT
GSE79306_deseq_E175_brain_EC_vs_E175_brain_total.txt.gz 2.1 Mb (ftp)(http) TXT
GSE79306_deseq_E175_wt_brain_EC_vs_E175_KO_brain_EC.txt.gz 2.1 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap