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Series GSE25002 Query DataSets for GSE25002
Status Public on Feb 01, 2014
Title Expression data from Saccharomyces cerevisiae expressing Candida albicans Nik1p comparing osmotic stress and fludioxonil treatment
Platform organisms Schizosaccharomyces pombe; Saccharomyces cerevisiae
Sample organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary Fungal group III histidine kinases are the molecular targets of some classes of fungicides. In contrast to the yeast Saccharomyces cerevisiae, the fungal pathogen Candida albicans possesses a group III histidine kinase, CaNik1p, also called Cos1p. To investigate the function of CaNIK1, the gene was expressed in S. cerevisiae. The transformants became susceptible to antifungal compounds to which the wild-type strain is resistant. The susceptibility was related to the activation of the MAP kinase Hog1p of the osmotic stress response pathway. Gene expression analysis revealed a strong overlap of the responses to osmotic stress and to fludioxonil at early time points. While the response to fludioxonil persisted, the response to osmotic stress was diminished with time.
 
Overall design S. cerevisiae expressing Candida albicans Nik1p were treated with 10 µg/ml fludioxonil. As a comparison, another culture of S. cerevisiae expressing Candida albicans Nik1p was treated with 1 M sorbitol to induce osmotic stress response. One culture remained untreated as a control. From all cultures, samples were taken after a duration of 15, 30 and 60 min.
 
Contributor(s) Buschart A, Gremmer K, van den Heuvel J, Müller P, Bilitewski U
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Submission date Oct 28, 2010
Last update date Jun 06, 2024
Contact name Anna Buschart
E-mail(s) anna.buschart@helmholtz-hzi.de
Organization name Helmholtz-Centre for Infection Research
Department BiSA
Street address Inhoffenstr. 7
City Braunschweig
ZIP/Postal code 38124
Country Germany
 
Platforms (1)
GPL2529 [Yeast_2] Affymetrix Yeast Genome 2.0 Array
Samples (33)
GSM614229 Transformant yeast untreated 15 min rep1
GSM614230 Transformant yeast untreated 15 min rep2
GSM614231 Transformant yeast untreated 30 min rep1
Relations
BioProject PRJNA134359

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE25002_RAW.tar 35.3 Mb (http)(custom) TAR (of CEL, TXT)
Processed data included within Sample table

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