Extended Data Fig. 4: Structural comparison of TAS2R14 with TAS2R46 and class A GPCRs.
From: Bitter taste TAS2R14 activation by intracellular tastants and cholesterol
(a) Schematic representation of constructs design for the G proteins used in the complex structure determination. Red * label indicates the different residues in α5 helix between Gαg and Gαi1. (b) Dose–response curves of Gαi1-Gβγ, Gαi2-Gβγ or Gαi3-Gβγ dissociation in TAS2R14-overexpressing HEK293 cells in response to FFA. Values are represented ad mean ± s.e.m. of 3 independent experiments (n = 3). (c) Structural comparison of AA-TAS2R14 and strychnine-TAS2R46 in side view (c), extracellular view (d) and intracellular view (e). Strychnine is an agonist for TAS2R46. The conformational changes are indicated by red arrows. (f) The sequence alignment of conserved residues in TAS2Rs (TAS2R14 and TAS2R46) and class A GPCRs (β2AR and CB1). (g) The 3D structural comparison of TAS2R14 with TAS2R46 and β2AR in active states. The conserved residues are labelled for TMs1-7 excluding TM4.