Extended Data Fig. 9: The BRET assay of FFA activated TAS2R14 in Gg and Gi pathways and compound 28.1 in Gg pathway.
From: Bitter taste TAS2R14 activation by intracellular tastants and cholesterol
(a-c) Dose–response curves of single mutations in pocket 1 (a), pocket 2 (b) and double or triple mutations in pocket 1 (c) in TAS2R14 in response to stimulation with FFA by Gαg-Gβγ dissociation assay. Values are represented as mean ± s.e.m. of 3 independent experiments (n = 3). (d-e) Dose–response curves of single mutations in pocket 1 (d) and pocket 2 (e) in TAS2R14 in response to stimulation with FFA by Gαi-Gβγ dissociation assay. Values are represented as mean ± s.e.m. of 3 independent experiments (n = 3). (f) Dose–response curves of single mutations in pocket 2 in TAS2R14 in response to stimulation with compound 28.1 by Gαg-Gβγ dissociation assay. Values are represented as mean ± s.e.m. of 3 independent experiments (n = 3).