Extended Data Fig. 6: Dose responses curves of key residues mutations in ligand binding pockets and receptor activation for aristolochic acid (AA), flufennamic acid (FFA) and 28.1 in TAS2R14 in calcium mobilization assay.
From: Bitter taste TAS2R14 activation by intracellular tastants and cholesterol
(a-e) Dose–response curves of key residues mutations in TAS2R14 in response to stimulation with AA in calcium mobilization assay. Data are mean ± s.e.m. from 3 independent experiments (n = 3). (f-g) Dose–response curves of key residues mutations in TAS2R14 in response to stimulation with FFA in calcium mobilization assay. Data are mean ± s.e.m. from 3 independent experiments (n = 3). (k) Dose–response curves of key residues mutations of pocket 2 in TAS2R14 in response to stimulation with compound 28.1 in calcium mobilization assay. Data are mean ± s.e.m. from 3 independent experiments (n = 3). Also see Supplementary tables 1–3.