Extended Data Fig. 1: Schematic of an in vitro integration assay and effects of IstB ATPase mutants and nucleotides on the integration reaction.
From: Molecular basis for transposase activation by a dedicated AAA+ ATPase
a, Pre-cleaved donor DNA includes the sequence of the transposon end (orange; TS, transferred strand; NTS, non-transferred strand). The donor duplex, containing a terminal CA dinucleotide with a reactive 3’-OH (green), can be integrated by IstA into a supercoiled target plasmid (black) in the presence of IstB. The reaction produces relaxed and linearized plasmids as result of half or concerted integration, respectively. b, Representative native agarose gel of the effect of IstB mutants and nucleotides on the integration reaction (n = 3 independent experiments). For gel source data, see Supplementary Fig. 1.