Extended Data Fig. 5: Cryo-EM imaging and image processing pipeline of the holo-transpososome complex.
From: Molecular basis for transposase activation by a dedicated AAA+ ATPase
a, Representative cryo-EM micrograph from the reconstituted holo-transpososome sample. Scale bar (white, bottom left) represents 100 nm. The micrograph shown is an example image from a combined dataset consisting of 36,276 micrographs. b, Image processing workflow used to analyze the cryo-EM data. c, Fourier Shell Correlation (FSC) of the cryo-EM density map. d, Angular distribution plot. e, Local resolution of the sharpened map. f, Comparison of the unsharpened map at 2 different thresholds (0.00327 and 0.00144 in gold and grey, respectively) highlights the flexibility present at the distal sites of the transposase and IstB.