Extended Data Fig. 10: Depletion of Wuhan-1 NTD, RBD, S2, and spike-reactive antibodies from serum.
From: Imprinting of serum neutralizing antibodies by Wuhan-1 mRNA vaccines
Antibody binding to Wuhan-1 NTD, RBD, S2, or spike proteins of pre- and post-boost sera of 1273/1273/1273/222/815-vaccinated individuals without (a) or with (b) a history of SARS-CoV-2 infection after pre-clearing with empty beads (no Dp), Wuhan-1 N-terminal domain protein-loaded beads (NTD Dp), RBD-loaded beads (RBD Dp), S2 protein-loaded beads (S2 Dp), a 1:1 mixture of RBD-loaded and S2-loaded beads (RBD & S2 Dp), or spike-loaded beads (spike Dp) (dotted lines show the LOD; values at the LOD are plotted slightly below the LOD for visualization; individuals that became infected (anti-N antibody negative before the fourth dose and positive before the fifth dose) are indicated with red borders; all other individuals in b are anti-N positive before the fourth dose). Binding to Wuhan-1 NTD, RBD, and S2 was interpolated from SARS2-57, SARS2-38, and CV3-25 standard curves, respectively. c, Serum neutralizing activity of infection-naïve post-boost sera pre-cleared with empty beads (no Dp), Wuhan-1 RBD-loaded beads (RBD Dp), or a mixture of Wuhan-1 RBD-loaded and S2-loaded beads (RBD & S2 Dp) against SARS-CoV-1 pseudovirus (data also shown in Fig. 5e, third panel from left) (connecting lines represent sera from the same individual). Statistical analysis: two-sided Wilcoxon signed-rank test (P values are shown).