Extended Data Fig. 6: Imprinting effects on MBCs after boosting with bivalent mRNA-1273.214 vaccine.
From: Imprinting of serum neutralizing antibodies by Wuhan-1 mRNA vaccines
a, Scheme of immunizations and blood draws. b, Gating scheme for identification of spike-binding MBCs in blood. c-d, Study participants (n = 20) who previously received three doses of mRNA-1273 were boosted with mRNA-1273.214 (Wuhan-1–BA.1 bivalent vaccine), and peripheral blood mononuclear cells (PBMCs) were obtained on days 0 (pre-boost), 28 (post-boost), and 90 (post-boost). c, Representative flow cytometry dot plots showing MBC specificities for pairs of fluorescently labeled WA-1/2020 and BA.1 spike proteins. Quadrant frequencies represent percentage of total MBCs. d, Fractions of spike-binding MBCs with indicated specificities (WA-1+BA.1+, left; WA-1+BA.1−, middle; or WA-1−BA.1+, right) at days 0, 28, and 90 (numbers at the top denote median values). d, Kruskal-Wallis ANOVA with Dunn’s post-test (P values are shown).