Extended Data Fig. 9: CTLA-4+ ILC3s reduce co-stimulatory molecules and enhance PD-L1 on myeloid cells in response to IL-23.
From: CTLA-4-expressing ILC3s restrain interleukin-23-mediated inflammation
Sorted ILC3s and myeloid cells from the large intestine of C57BL/6 mice were co-cultured with recombinant IL-23 in presence or absence of indicated blocking antibodies. Flow cytometry plots with graph of MFI of (a) CD80 and (b) CD86 expression on myeloid cells gated as CD11c+ MHCIIhi. c, Flow cytometry plots with graph of free PD-L1 frequency on myeloid cells gated as CD11c+ MHCIIhi. d, Gating strategy to analyze T cells, myeloid cells and ILC3s in human samples. Data in a-c are representative of two independent experiments with similar results (n = 4 mice) and shown as Mean ± s.d. The statistics were calculated by one-way ANOVA with Dunnett’s multiple comparisons.