Extended Data Fig. 6: Steady state analysis of Ncr1cre Ctla4f/f mice.
From: CTLA-4-expressing ILC3s restrain interleukin-23-mediated inflammation
a, Flow cytometry plots of NKp46+ cells in large intestine lamina propria of naïve and C. rodentium infected C57BL/6 mice (n = 4 mice). Lineage 1, CD11b, CD11c and B220; lineage 2, CD3ε, CD5, CD8α, and TCRγδ. b, UMAP plot of scRNA-seq data of IL-23R+ immune cells from the small intestine of IL-23R-eGFP mice showing Ctla4 expression in different clusters. The large intestine of Ncr1cre Ctla4f/f and Ctla4f/f were analyzed at steady state and graph displaying the frequency of (c) ILC3s (percentage of Lin- CD90+ CD127+) and (d) IL-22+ ILC3s (percentage of total ILC3s). e, Graph of TH17 cells frequency (percentage of CD4+ T cells). Flow plots with graph displaying the frequency of (f) Treg cells (percentage of CD4+ T cells) and (g) neutrophils gated as CD11b+Ly6G+ cells (percentage of CD45+ cells). Data in a,c,d,e,f and g are representative of two or three independent experiments with similar results (n = 5 mice) and shown as Mean ± s.d. The statistics in c-g were determined by Mann–Whitney U-test (unpaired, two-tailed).