Extended Data Fig. 10: Deletion of Pdcd1 suppresses tumor growth and promotes a pro-inflammatory, glycolytic phenotype in macrophages.
From: Obesity induces PD-1 on macrophages to suppress anti-tumour immunity
a, Tumor weight on Day 14 following 1×106 MC38 cells injected subcutaneously into WT or Pdcd1−/− age matched mice (n = 8-10mice). b, Percent phrodo+ of peritoneal macrophages from WT or Pdcd1−/ − tumor-exposed mice c, Percent positive and MFI of MHCII of peritoneal macrophages d) Percent positive and MFI of CD80 of peritoneal macrophages e, MFI of 2-NBDG in peritoneal macrophages following 45 min exposure to 2-NDBG. f-g, Tumor diameter over time and tumor weight on Day 14 following 5×106 MC38 cells injected subcutaneously into WT or Pdcd1−/ − age matched mice (n = 3-5mice). h-j, Frequency and MFI of MHCII, CD86 and Glut1 expression on TAMs from WT or Pdcd1−/ − mice. k, OCR and ECAR measurements over time from MitoStress Test of BMDMs isolated from WT or Pdcd1−/ − mice stimulated for 24 h with IFN-g (M1), IL-4 (M2), or 50% Tumor Conditioned Media (TCM). l, ECAR measurement over time from GlycoStress test of BMDMs with corresponding Glycolysis calculation. Data points in f-g represent 6 biological replicates run in triplicate. P values were calculated using an unpaired two-tailed t-test; ns= p > 0.05, *p ≤ 0.05, **p ≤ 0.05).