Extended Data Fig. 2: ELISA screening allows set for down selection of candidates from Set 2 (n = 204), for down-stream characterization.
From: Computationally restoring the potency of a clinical antibody against Omicron
a-d, Parental mAb COV2-2130 (orange circles; 3 technical replicates) and positive control mAb S309 24 (magenta squares; 3 technical replicates) serve as references for computationally designed mAbs (black curves). Purple triangles are 2130-1-114-112; blue diamonds are 2130-1-0104-024. Each designed antibody had a single measurement (n = 1) at each concentration. All curves are 4-parameter logistic fits, produced in GraphPad Prism. Target antigens are RBD from (a) wild type WA1/2020, (b) Omicron BA.1 (Acro), which is biotinylated, (c) Omicron BA.1, and (d) Omicron BA.1.1. For the biotinylated antigen in (b) (Acro Biosystems, cat. SPD-C82E4), an additional coat and wash cycle was required to prepare the ELISA plate with streptavidin.