Extended Data Fig. 5
From: Tumour-selective activity of RAS-GTP inhibition in pancreatic cancer
(a-e) KPC mice (from Fig. 5) were treated with Vehicle (n = 11) or RMC-7977 (50 mg/kg, n = 33) for indicated time, with tissues collected either at 4 or 24 h post last dose (a) qRT-PCR analysis was run on RNA isolated from KPC tumors. Plots show relative expression of five MAPK pathway signature genes at indicated timepoints. Results were compared by two-tailed Student’s unpaired t-test (*, p < 0.05; **, p < 0.01; p < 0.001; ****, p < 0.0001). Error bars indicate ±s.d. (b) Representative IHC images of KPC colon and skin collected at endpoint and stained for CC3. Scale bars = 100 μm. (c) Quantification of CC3 IHC staining in colon, and skin. (d) Representative IHC images of KPC colon and skin collected at endpoint and stained for Cyclin A2. Scale bars = 100 μm. (e) Quantification of Cyclin A2 IHC staining in colon and skin. (c,e) Quantification of IHC images was based on 10 fields of view (light shade), averaged per tissue section (dark shade) and means were compared by two-tailed Student’s unpaired t-test (*, p < 0.05; **, p < 0.01). Error bars indicate ±s.d.