Extended Data Fig. 4
From: Tumour-selective activity of RAS-GTP inhibition in pancreatic cancer
(a) KPC mice (from Fig. 5) treated with Vehicle (n = 9) or RMC-7977 (50 mg/kg, p.o., q.o.d., n = 13) until endpoint criteria were met. Tolerability of RMC-7977 in KPC mice as assessed by percent animal body weight change from baseline over time. (b,c) KPC mice (from Fig. 5) were treated with Vehicle (n = 10) or RMC-7977 (n = 31) for indicated time, with tissues collected either at 4 or 24 h post last dose. (b) Representative IHC images of KPC tumors collected at indicated timepoints, stained for phospho-ERKT202/Y204, phospho-S6S235/236 and phospho-S6S240/244. Scale bars = 100 μm. (c) Representative IF images of KPC tumors collected at indicated timepoints, stained for phospho-ERKT202/204 (left panel, red), phospho-S6S235/236 (right panel, red) and CK19 (green), and counterstained with DAPI (blue). Scale bars = 50 μm.