Extended Data Fig. 5: Tumorigenesis in the mini-colon leads to enhanced RTK signalling promoting growth factor independence.
From: Spatiotemporally resolved colorectal oncogenesis in mini-colons ex vivo
a, Gene interaction network of the overlapping genes that are upregulated in AKP lines with high-proliferation potential in BM (“in vivo AKP”, “mini-colon AKP” #v) when compared to low-growth counterparts (“organoid AKP”, “mini-colon AKP” #i). Network hubs are highlighted with circles. b, Brightfield images of “mini-colon AKP” #v organoids cultured for 3 days with the indicated media and compounds. Scale bar, 200 μm. c, Metabolic activity (measured using resazurin) of “mini-colon AKP” #v organoids cultured in the indicated conditions. *P = 0.0236; ***P < 0.0001 (two-way ANOVA and Sidak’s multiple comparisons test; n = 4 cultures for infigranib and 8 for the rest of conditions). d, Brightfield images of “organoid AKP” cells cultured for 3 days in BM with the indicated growth factors. Scale bar, 200 μm. e, Metabolic activity (measured using resazurin) of “organoid AKP” cells cultured in the indicated conditions. ***P < 0.0001 (two-way ANOVA and Sidak’s multiple comparisons test; n = 8 cultures for each condition). In c and e, each point represents one well of organoids and data represent mean ± SEM.