Extended Data Fig. 3: Oncogenic mutations induce full blown tumours in mini-colons.
From: Spatiotemporally resolved colorectal oncogenesis in mini-colons ex vivo
a, Hematoxylin and eosin staining of a mini-colon tumour section. Scale bar, 25 μm. b, Time-course growth of tumours produced by cells derived from mini-colon tumours upon subcutaneous transplantation in immunodeficient mice (n = 5 mice). As a reference, bona fide cancer cells from primary colon tumours are included. c, Image of the tumours at the endpoint of the experiment shown in panel b. d, Hematoxylin and eosin stainings of sections from the indicated tumour types. Zoomed-in areas (right) are indicated with a dashed square (left). Scale bar, 100 μm. e, Hematoxylin and eosin stainings of sections from mini-colon AKP implant tumours showing the presence of invading cancer cells (left, black arrowheads) and areas of cellular atypia (right, white arrowhead). Scale bar, 200 μm. f, Electrophoretic separation of PCR-amplified KRASLSL locus in the indicated samples. See Methods for more details on PCR design. g, Whole exome sequencing coverage in the indicated loci and cells. Missing exons in recombined cells are indicated. h, Brightfield images of mini-colons of the indicated genotypes 23 days after blue light exposure. Neoplastic and tumour structures are indicated with black and white arrowheads in A and AK mini-colons, respectively. By that time tumours have extended throughout the whole mini-colon tissue in the case of the AKP model, forming a dense mass of cancer cells. Scale bar, 75 μm.