Extended Data Fig. 3: Parallel regulation of metabolism and inflammation by glucocorticoids.
From: Metabolic rewiring promotes anti-inflammatory effects of glucocorticoids
(a) ELISA-based quantification of the indicated cytokines in the supernatants human monocytes-derived macrophages from healthy donors under normoxia and hypoxia (1% O2) and treated as indicated with vehicle (Ctrl), dexamethasone (GC, 100 nM) and LPS and IFN-γ (100 ng/ml and 20 ng/ml, respectively) (n = 12). (b) Quantification of indicated parameters derived from a mito stress test and a glycolysis stress test of bone marrow-derived macrophages (BMDMs) treated with Ctrl, GC and LPS in the presence of a vehicle or roxadustat (RXD) (10 µM) for 24 h (n = 8). Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured. (c) ELISA-based quantification of the indicated cytokines in the supernatants of BMDMs treated with Ctrl, GC and LPS in the presence of a vehicle or UK5099 (10 µM) as indicated (n = 6). Data are presented as mean + SEM. One-way ANOVA with Tukey’s multiple comparison test (a-c).