Extended Data Fig. 2: Regulation of TCA cycle metabolism by glucocorticoids.
From: Metabolic rewiring promotes anti-inflammatory effects of glucocorticoids
(a) Mass spectrometry-based analysis of U-13C glucose carbon tracing in bone marrow-derived macrophages (BMDMs) treated with vehicle (Ctrl), dexamethasone (GC, 100 nM) and LPS (100 ng/ml). Relative U-13C exchange rate (RelER) for the indicated metabolites and the number of exchanged carbon atoms (M0 to M6). (b) Extracellular acidification rate (ECAR) of a glycolysis stress test, with quantified glycolysis and glycolytic capacity of fetal liver-derived macrophages from Nls+/+ and Nlsmut mice treated with vehicle, GC and LPS for 24 h (n = 8). (c) Immunofluorescence imaging of BMDMs treated with Ctrl, GC and LPS for 24 h and stained with MitoTracker Red and an antibody directed against the glucocorticoid receptor. (d) Immunofluorescence microscopy of PDH-X protein in BMDMs upon stimulation with Ctrl, GC and LPS for 6 h. Fluorescence signal rendering for PDH-X and MitoTracker is represented in the lower right panel, with quantification in Fig. 2g. (e) Western blot analysis of pyruvate dehydrogenase (PDH) protein levels in BMDMs treated with Ctrl, GC, 100 nM and LPS for 6, 12 and 24 h. Data are presented as mean + SEM. One-way ANOVA with Tukey’s multiple comparison test (b).