Extended Data Fig. 1: Cryo-EM data processing for the TAS2R14–Gi1–scFv16 complex.
From: Bitter taste receptor activation by cholesterol and an intracellular tastant
a, Workflow of cryo-EM data processing for TAS2R14–Gi1–scFv16 complex. Masks used for focused classification and local refinement were colored pink and magenta, respectively. The locally refined map is rotated by 90° based on the y-axis from the globally refined map for clarity. b, A representative motion-corrected micrograph imaged at a nominal 81,000 × magnification. The experiment was repeated three times with similar results. c, Representative 2D class averages without alignment. d, Map-to-model FSC curve calculated by mtriage in Phenix software. e-j, Representative local resolution (e), Gold-standard Fourier shell correlation (GSFSC) curves adopted from cryoSPARCv4 (f), and particle angular distributions (g) for globally refined map (e-g) and locally refined map (h-j).