Extended Data Fig. 7: Verification of Poisson dominated accuracy for dCERS.
From: Digital colloid-enhanced Raman spectroscopy by single-molecule counting
(a) The number of positive voxels increases with an increase in the total number of voxels acquired per dCERS test for crystal violet concentrations of 10−11 to 10−15 M crystal violet (from top to bottom) measured using Hya-Ag colloids. (b) The number of positive voxels increased with an increase in the concentration of 4-NBT, hemoglobin, A12 and glucose (from top to bottom) measured using Hya-Ag colloids. The total number of measured voxels per dCERS test was 5,400 for 4-NBT and 1,200 for the others. The gray bars show the mean number of positive voxels with the error bars indicating the standard deviations from 3 independent tests. The relative standard deviation (RSD, red dots) is calculated as the ratio of the standard deviation to the mean of the positive count, which agrees with the Poisson estimation (\(\frac{1}{\sqrt{N}}\), blue lines) from the mean positive counts (N).