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Confocal microscopy is a powerful and versatile technique for studying biological phenomena on scales ranging from tissue-level structures to subcellular details. Conducting microscopy experiments with living samples often yields valuable, physiologically relevant data.

However, acquiring clear images can be challenging due to factors such as sample photosensitivity, the relatively fast timescales of biological processes, and the inescapable diffraction limit of light microscopy. Inadequate image quality prevents subsequent analysis and can ultimately hinder research.

Image scanning microscopy (ISM), an alternative to traditional confocal microscopy, was developed to enhance image resolution and contrast to obtain a clearer picture of any given sample. The technique hinges on the acquisition of additional spatial information about the sample, which enables direct improvements to image quality without detrimental increases in laser illumination power or acquisition time.

This webcast will describe the implementation and advantages of ISM using an array of highly sensitive single photon avalanche photodiodes (SPADs). 

You will learn:

• How image scanning microscopy (ISM) enhances image quality without compromising other parameters like acquisition time or illumination intensity
• Which applications are particularly well-suited for ISM
• Unique advantages of conducting ISM with a photon-counting detector array