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Accurate, quantitative, high-throughput multi-omics measurements can fill longstanding gaps in basic biochemistry and elucidate confounding disease processes.

This webcast will highlight how new methodologies using mass spectrometry enable deep proteome sequencing and population-scale biological discovery.

In recent work with a human cell library of CRISPR-mediated gene knockout, simultaneous measurements of proteins and metabolites identify a mutation in a gene that underlies a previously unresolved mitochondrial disorder.

Advances in deep proteome sequencing are particularly important for the discovery and quantitative comparison of all unique proteoforms – including those that arise from alternative splicing events and single nucleotide polymorphisms (SNPs). With this methodology, over one million unique human peptides from 17,717 protein groups can be identified, with a median sequence coverage of nearly 80 percent. Multi-omics analyses from these data provide large-scale evidence that most alternatively spliced isoforms are translated.

Further improvements with the novel Thermo Scientific Orbitrap Astral mass spectrometer enable detection of nearly 100,000 unique phosphorylation sites within only 12 hours of analysis, measurement of a near-complete human proteome in under one hour, and provide a route to routine deep proteome sequencing.

• Study biological systems using multi-omics approaches
• Perform deep proteome sequencing using mass spectrometry
• Apply novel mass spectrometry technology to solve biological questions