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The genome is the full genetic complement of an organism, encoded in either DNA or, in many viruses, RNA. It includes both the genes and non-coding sequences.
Pacific Biosciences (PacBio) High Fidelity (HiFi) long-read sequencing usually requires a relatively high amount of DNA input ( > 1 µg). Here the authors develop LILAP, a Low-Input (100 ng), Low-cost, and Amplification-free library-generation method for PacBio sequencing, enabling the generation of two high-quality individual fly genomes.
In this Journal Club article, Laura Ross discusses several seminal papers that describe the discovery of germline-specific chromosomes and paternal genome elimination, striking examples of non-Mendelian genetics.
Logsdon et al. report the second complete sequence of all centromeres from a single human genome, enabling comparative analyses of the variation in tandemly repeating α-satellite DNA.
Bruce Budowle and Antti Sajantila reflect on how short tandem repeats (STRs) became the primary markers of forensic genetics, including for developing investigative leads in criminal cases and humanitarian efforts.