Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain
the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in
Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles
and JavaScript.
A DNA damage checkpoint is a pause in the cell cycle that is induced in response to DNA damage to ensure that the damage is repaired before cell division resumes. Proteins that accumulate at the damage site typically activate the checkpoint and halt cell growth at the G1/S or G2/M boundaries.
The TRAIP E3 ubiquitin ligase is essential for genome integrity, mutations lead to primordial dwarfism in patients. Here, the authors show that TRAIP degradation in S-phase, results in cell arrest due to DNA damage caused by replication-transcription conflicts.
Cryo-EM structures of the yeast 9-1-1 checkpoint clamp in complex with the Rad24-RFC clamp loader and a DNA substrate explain how 9-1-1 is recruited to DNA junctions with recessed 5′ ends and reveal the mechanism of sliding clamp loading.
The expression of two DNA repair factors improves the recombination of single-stranded oligodeoxynucleotides with Cas9-induced double-strand breaks, facilitating precise and efficient gene editing.
DNA damage-induced histone degradation results in decreased nucleosome occupancy, which promotes homologous recombination by enhancing the dynamicity of chromatin.