Extended Data Fig. 2: Analysis of the closed conformation of the Mis12C heads.
From: Structure of the human KMN complex and implications for regulation of its assembly
(a) Comparison of the closed conformation of the heads observed in this study and the open conformation of the heads observed in a previous crystal structure of the complex of Mis12C with CENP-C (PDB 5LSK; ref. 1). In the central superposition, the movement that sways the head2 out of position is marked by a black arrow. The red arrowheads indicate an outward movement of the helices lining the cleft that separates the DSN1 and MIS12 helices when CENP-C binds. (b) AF2 model of the interface between a positively-charged regulatory loop of DSN1 and the negatively charged head1 domain (with contributions from NSL1 and DSN1 in head2).