Extended Data Fig. 3: KNL1 RWD domains and hydrodynamic analyses of mutants.
From: Structure of the human KMN complex and implications for regulation of its assembly
(a) Definition of secondary structure of the two RWD domains of the KNL1 C-terminal region, as originally defined in reference2. (b) Comparison of the interaction of the NSL1 C-terminal tail with KNL1 as emerging from our current and previous work2. The model of the NSL1 chain in this representation includes extensions, built by AlphaFold2, to the helical segment shown in Fig. 2a. Details in Methods and main text. (c-f) Size-exclusion chromatography elution profiles for the indicated wild type and mutant species. (c) KNL1 was analyzed on a HiLoad 16/600 Superdex 75 column, (d) Mis12C and (e) SPC24/SPC25 species were analyzed on a HiLoad 16/600 Superdex 200 column. (f) ZWINT was analyzed on a Superdex 75 10/300 column.