We used high-throughput transposon screens to examine the responses of generalist and host-adapted Salmonella enterica serovars to 25 stress conditions that recapitulate key stages of human infection. We identified and characterized numerous typhoid-specific gene networks, revealing a role for specific pseudogenes in shaping bacterial fitness outcomes.
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References
Wang, B. X., Butler, D. S., Hamblin, M. & Monack, D. M. One species, different diseases: the unique molecular mechanisms that underlie the pathogenesis of typhoidal Salmonella infections. Curr. Opin. Microbiol. 72, 102262 (2023). A review article that presents a summary of genetic differences between non-typhoidal and typhoidal Salmonella.
McClelland, M. et al. Comparison of genome degradation in Paratyphi A and Typhi, human-restricted serovars of Salmonella enterica that cause typhoid. Nat. Genet. 36, 1268–1274 (2004). This paper reports genomic comparisons between S. Typhiand S. Paratyphi A.
Holt, K. E. et al. High-throughput sequencing provides insights into genome variation and evolution in Salmonella Typhi. Nat. Genet. 40, 987–993 (2008). This paper uses bioinformatics to more deeply characterize the genomes of S. Typhi.
Wetmore, K. M. et al. Rapid quantification of mutant fitness in diverse bacteria by sequencing randomly bar-coded transposons. mBio 6, 1–15 (2015). This paper explains the principle and development of Rb-Tn-seq.
Leshchiner, D. et al. A genome-wide atlas of antibiotic susceptibility targets and pathways to tolerance. Nat. Commun. 13, 1–17 (2022). This paper applies SAFE to systematically study the fitness of a Gram-positive pathogen.
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This is a summary of: Wang, B. X. et al. High-throughput fitness experiments reveal specific vulnerabilities of human-adapted Salmonella during stress and infection. Nat. Genet. https://doi.org/10.1038/s41588-024-01779-7 (2024).
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High-throughput fitness screens link genes to unique phenotypes in human-restricted Salmonella. Nat Genet 56, 1053–1054 (2024). https://doi.org/10.1038/s41588-024-01780-0
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DOI: https://doi.org/10.1038/s41588-024-01780-0
