The majority of the human non-coding genome remains poorly studied. A user-friendly genome-wide screening system composed of thousands of paired single-guide RNAs for the deletion of non-coding regions revealed key functions of many non-coding elements in cell growth and cell differentiation and in cellular response to drugs.
This is a preview of subscription content, access via your institution
Access options
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
$29.99 / 30 days
cancel any time
Subscribe to this journal
Receive 12 digital issues and online access to articles
$99.00 per year
only $8.25 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Nurk, S. et al. The complete sequence of a human genome. Science 376, 44–53 (2022). This paper provides the latest human genome sequence.
Pang, B., van Weerd, J. H., Hamoen, F. L. & Snyder, M. P. Identification of non-coding silencer elements and their regulation of gene expression. Nat. Rev. Mol. Cell Biol. 24, 383–395 (2023). This review article highlights the various NCREs, including silencers.
Dimitrieva, S. & Bucher, P. UCNEbase—a database of ultraconserved non-coding elements and genomic regulatory blocks. Nucl. Acids Res. 41, D101–D109 (2012). This paper catalogues the ultraconserved non-coding elements.
Yip, K. Y. et al. Classification of human genomic regions based on experimentally determined binding sites of more than 100 transcription-related factors. Genome Biol. 13, R48 (2012). This paper presents the ENCODE-predicted enhancers in K562 cells.
Pang, B. & Snyder, M. P. Systematic identification of silencers in human cells. Nat. Genet. 52, 254–263 (2020). This paper reports the identification of NCREs—silencers.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This is a summary of: Li, Y. et al. Genome-wide Cas9-mediated screening of essential non-coding regulatory elements via libraries of paired single-guide RNAs. Nat. Biomed. Eng. https://doi.org/10.1038/s41551-024-01204-8 (2024).
Rights and permissions
About this article
Cite this article
Paired CRISPR screening libraries for studying the function of the non-coding genome at scale. Nat. Biomed. Eng (2024). https://doi.org/10.1038/s41551-024-01215-5
Published:
DOI: https://doi.org/10.1038/s41551-024-01215-5
