Degradome sequencing of semi-active DCL1 mutants separates true miRNAs from DCL1-independent small RNAs in Arabidopsis thaliana and illustrates the processing pattern of 147 pri-miRNAs. In parallel, DMS-MaPseq decodes the in vivo secondary structures of pri-miRNAs, enabling a better understanding of cleavage modes and of the impact of DCL1 cofactors on cleavage.
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References
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This is a summary of: Yan, X. et al. Parallel degradome-seq and DMS-MaPseq substantially revise the miRNA biogenesis atlas in Arabidopsis. Nat. Plants https://doi.org/10.1038/s41477-024-01725-9 (2024).
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Exploring the landscape of miRNA production and the structural rules that shape it. Nat. Plants (2024). https://doi.org/10.1038/s41477-024-01728-6
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DOI: https://doi.org/10.1038/s41477-024-01728-6
