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Real-Time Visualization of Cholesterol Trafficking in Human Granulosa Cells Using Confocal Live Cell Microscopy as a Tool to Study the Novel Role of Autophagy in Sex Steroid Synthesis
Autophagy is an evolutionarily conserved process that aims to maintain the energy homeostasis of the cell by recycling long-lived proteins and...
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Visualizing Lipophagy as a New Mechanism of the Synthesis of Sex Steroids in Human Ovary and Testis Using Immunofluorescence Staining Method
Immunofluorescence, a transformative tool in cellular biology, is employed to dissect the intricate mechanisms of cholesterol trafficking in human...
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Regeneration of Volumetric Muscle Loss Using MSCs Encapsulated in PRP-Derived Fibrin Microbeads
Volumetric muscle loss (VML) is one of the major types of soft tissue injury frequently encountered worldwide. In case of VML, the endogenous...
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Measurement of Myonuclear Accretion In Vitro and In Vivo
Adult skeletal muscle stem cells (MuSC) are the regenerative precursors of myofibers and also have an important role in myofiber growth, adaptation,...
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Isolation of Murine Neonatal and Adult Osteomacs to Examine Their Role in the Hematopoietic Niche
Maintenance of hematopoietic stem cell (HSC) function is an orchestrated event between multiple cell types, and crosstalk between these cell types is...
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Microfluidic Chip Fabrication for Tumor Cell 3D Culture Based on Microwell Arrays
Compared with traditional 2D cell culture, 3D cell culture more closely resembles the original state of cells in vivo and enables the establishment...
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Methods for Generating Self-Organizing Human Patterned Heart Organoids Using Pluripotent Stem Cells
Organoids derived from pluripotent stem cells exhibit notable similarities to organ development in vitro. Nonetheless, cardiac organoids generated to...
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Evaluation of Autophagy in Conjunctival Fibroblasts
Vernal keratoconjunctivitis (VKC) is a serious eye allergy characterized by poorly understood pathogenic mechanisms and a lack of effective...
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Rat Ductal Cell–Derived Differentiation into Islet-Like Cells
Regenerative medicine investigates the conversion of pancreatic ductal cells into functional islet cells, offering innovative treatments for...
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Identification of De Novo Dividing Stem Cells
The ability to alternate between quiescent and proliferating states is a remarkable feature of many types of somatic stem cells. The balance between...
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Quality Control in Human Adipose-Derived Stromal/Stem Cells and Tissue Engineering Fat Models for Aging Studies
Adipose tissue is recognized not only as an endocrine organ but also as a reservoir for adipose-derived stromal/stem cells (ASCs). ASCs have...
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Light-Sheet Microscopy Enables Three-Dimensional Fluorescence Imaging and Live Imaging of Satellite Cells on Skeletal Muscle Fibers
The ex vivo myofiber culture system has proven to be a useful methodology to explore the biology and behavior of satellite cells within their niche...
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Proteomic Methods to Study Autophagy in Skin Exposed to Pollutants
Autophagy refers to the natural cellular process by which cells degrade and recycle their own damaged or dysfunctional cellular components. It is an...
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Measuring Stress Phenotypes in Cryptococcus neoformans
Cryptococcus neoformans is an opportunistic human fungal pathogen capable of surviving in a wide range of environments and hosts. It has been...
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Quantification of C. neoformans Capsule Diameter
The polysaccharide capsule of Cryptococcus neoformans is the primary virulence factor and one of the most commonly studied aspects of this pathogenic...
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Fluorescence and Biochemical Assessment of the Chitin and Chitosan Content of Cryptococcus
The cell wall of the fungal pathogens Cryptococcus neoformans and C. gattii is critical for cell wall integrity and signaling external threats to the...
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Analysis of Cryptococcus Extracellular Vesicles
Extracellular vesicles (EVs)Extracellular vesicles (EVs) are produced by all domains of life. In fungal pathogens, they participate in...
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Genetic Transformation of Cryptococcus Species with Agrobacterium Transfer DNA
Transformation of foreign DNA into Cryptococcus species is a powerful tool for exploring gene functions in these human pathogens. Agrobacterium...
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In Vivo Modeling of Cryptococcus neoformans Infection and Collection of Murine Samples
In vivo models provide advantages to study the progression of disease and to identify potential biomarkers to detect and monitor infections. For the...
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Biolistic Transformation of Cryptococcus neoformans
Biolistic transformation of Cryptococcus neoformans is used as a molecular tool to genetically alter or delete targeted genes. The DNA is introduced...
