Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Apr;53(4):394-404.
doi: 10.1016/j.ymeth.2010.12.017. Epub 2010 Dec 23.

High-throughput mouse phenotyping

Hilary Gates  1 , Ann-Marie Mallon, Steve D M Brown; EUMODIC Consortium
Affiliations

High-throughput mouse phenotyping

Hilary Gates et al. Methods. 2011 Apr.

Abstract

Comprehensive phenotyping will be required to reveal the pleiotropic functions of a gene and to uncover the wider role of genetic loci within diverse biological systems. The challenge will be to devise phenotyping approaches to characterise the thousands of mutants that are being generated as part of international efforts to acquire a mutant for every gene in the mouse genome. In order to acquire robust datasets of broad based phenotypes from mouse mutants it is necessary to design and implement pipelines that incorporate standardised phenotyping platforms that are validated across diverse mouse genetics centres or mouse clinics. We describe here the rationale and methodology behind one phenotyping pipeline, EMPReSSslim, that was designed as part of the work of the EUMORPHIA and EUMODIC consortia, and which exemplifies some of the challenges facing large-scale phenotyping. EMPReSSslim captures a broad range of data on diverse biological systems, from biochemical to physiological amongst others. Data capture and dissemination is pivotal to the operation of large-scale phenotyping pipelines, including the definition of parameters integral to each phenotyping test and the associated ontological descriptions. EMPReSSslim data is displayed within the EuroPhenome database, where a variety of tools are available to allow the user to search for interesting biological or clinical phenotypes.

PubMed Disclaimer

Similar articles

  • EuroPhenome: a repository for high-throughput mouse phenotyping data.
    Morgan H, Beck T, Blake A, Gates H, Adams N, Debouzy G, Leblanc S, Lengger C, Maier H, Melvin D, Meziane H, Richardson D, Wells S, White J, Wood J; EUMODIC Consortium; de Angelis MH, Brown SD, Hancock JM, Mallon AM. Morgan H, et al. Nucleic Acids Res. 2010 Jan;38(Database issue):D577-85. doi: 10.1093/nar/gkp1007. Epub 2009 Nov 23. Nucleic Acids Res. 2010. PMID: 19933761 Free PMC article.
  • Mouse phenotyping.
    Fuchs H, Gailus-Durner V, Adler T, Aguilar-Pimentel JA, Becker L, Calzada-Wack J, Da Silva-Buttkus P, Neff F, Götz A, Hans W, Hölter SM, Horsch M, Kastenmüller G, Kemter E, Lengger C, Maier H, Matloka M, Möller G, Naton B, Prehn C, Puk O, Rácz I, Rathkolb B, Römisch-Margl W, Rozman J, Wang-Sattler R, Schrewe A, Stöger C, Tost M, Adamski J, Aigner B, Beckers J, Behrendt H, Busch DH, Esposito I, Graw J, Illig T, Ivandic B, Klingenspor M, Klopstock T, Kremmer E, Mempel M, Neschen S, Ollert M, Schulz H, Suhre K, Wolf E, Wurst W, Zimmer A, Hrabě de Angelis M. Fuchs H, et al. Methods. 2011 Feb;53(2):120-35. doi: 10.1016/j.ymeth.2010.08.006. Epub 2010 Aug 12. Methods. 2011. PMID: 20708688 Review.
  • Methods for the behavioural phenotyping of mouse mutants. How to keep the overview.
    Arndt SS, Surjo D. Arndt SS, et al. Behav Brain Res. 2001 Nov 1;125(1-2):39-42. doi: 10.1016/s0166-4328(01)00285-6. Behav Brain Res. 2001. PMID: 11682091
  • Accessing and mining data from large-scale mouse phenotyping projects.
    Morgan H, Simon M, Mallon AM. Morgan H, et al. Int Rev Neurobiol. 2012;104:47-70. doi: 10.1016/B978-0-12-398323-7.00003-3. Int Rev Neurobiol. 2012. PMID: 23195311 Review.
  • Introduction to the Japan Mouse Clinic at the RIKEN BioResource Center.
    Wakana S, Suzuki T, Furuse T, Kobayashi K, Miura I, Kaneda H, Yamada I, Motegi H, Toki H, Inoue M, Minowa O, Noda T, Waki K, Tanaka N, Masuya H, Obata Y. Wakana S, et al. Exp Anim. 2009 Oct;58(5):443-50. doi: 10.1538/expanim.58.443. Exp Anim. 2009. PMID: 19897927 Review.
See all similar articles

Cited by

See all "Cited by" articles

Publication types

MeSH terms

LinkOut - more resources