Dual RMCE for efficient re-engineering of mouse mutant alleles
- PMID: 20953177
- PMCID: PMC3576631
- DOI: 10.1038/nmeth.1521
Dual RMCE for efficient re-engineering of mouse mutant alleles
Abstract
We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of interest in an easy and highly efficient manner.
Figures
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Comment in
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The author file: Rolf Zeller and Javier Lopez-Rios.Nat Methods. 2010 Nov;7(11):861. doi: 10.1038/nmeth1110-861. Nat Methods. 2010. PMID: 21049587
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Resources for proteomics in mouse embryonic stem cells.Nat Methods. 2011 Feb;8(2):103-4. doi: 10.1038/nmeth0211-103. Nat Methods. 2011. PMID: 21278719 No abstract available.
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