The use of DNA transposons for cancer gene discovery in mice
- PMID: 20699138
- DOI: 10.1016/S0076-6879(10)77006-3
The use of DNA transposons for cancer gene discovery in mice
Abstract
Insertional mutagenesis in mice is a potent instrument for cancer gene discovery. Until recently, retroviruses were the main experimental tools in this field and application of insertional mutagenesis was limited to tissues for which these agents have tropism, namely hemopoietic cells and mammary epithelium. However, the field has been revolutionized and greatly expanded with the recent reanimation of the transposons, a highly flexible group of insertional mutagens first discovered in maize, which have now been adapted for use in mammalian cells. Transposons do not only extend the application of insertional mutagenesis to any tissue of choice, but also allow a more extensive and unbiased coverage of the genome, can be designed to selectively activate or inactivate genes, and are highly amenable to temporal and spatial control. This chapter gives an overview of the design and application of transposons to cancer gene discovery in mice.
2010 Elsevier Inc. All rights reserved.
Similar articles
-
Hopping around the tumor genome: transposons for cancer gene discovery.Cancer Res. 2005 Nov 1;65(21):9607-10. doi: 10.1158/0008-5472.CAN-05-3085. Cancer Res. 2005. PMID: 16266976 Review.
-
Recent translational research: oncogene discovery by insertional mutagenesis gets a new boost.Breast Cancer Res. 2006;8(1):102. doi: 10.1186/bcr1376. Epub 2006 Jan 16. Breast Cancer Res. 2006. PMID: 16469121 Free PMC article.
-
Cancer gene discovery in solid tumours using transposon-based somatic mutagenesis in the mouse.Nature. 2005 Jul 14;436(7048):272-6. doi: 10.1038/nature03681. Nature. 2005. PMID: 16015333
-
Mammalian mutagenesis using a highly mobile somatic Sleeping Beauty transposon system.Nature. 2005 Jul 14;436(7048):221-6. doi: 10.1038/nature03691. Nature. 2005. PMID: 16015321
-
Insertional mutagenesis in mice: new perspectives and tools.Nat Rev Genet. 2005 Jul;6(7):568-80. doi: 10.1038/nrg1638. Nat Rev Genet. 2005. PMID: 15995698 Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
