The functional annotation of mammalian genomes: the challenge of phenotyping
- PMID: 19689210
- DOI: 10.1146/annurev-genet-102108-134143
The functional annotation of mammalian genomes: the challenge of phenotyping
Abstract
The mouse is central to the goal of establishing a comprehensive functional annotation of the mammalian genome that will help elucidate various human disease genes and pathways. The mouse offers a unique combination of attributes, including an extensive genetic toolkit that underpins the creation and analysis of models of human disease. An international effort to generate mutations for every gene in the mouse genome is a first and essential step in this endeavor. However, the greater challenge will be the determination of the phenotype of every mutant. Large-scale phenotyping for genome-wide functional annotation presents numerous scientific, infrastructural, logistical, and informatics challenges. These include the use of standardized approaches to phenotyping procedures for the population of unified databases with comparable data sets. The ultimate goal is a comprehensive database of molecular interventions that allows us to create a framework for biological systems analysis in the mouse on which human biology and disease networks can be revealed.
Similar articles
-
Understanding mammalian genetic systems: the challenge of phenotyping in the mouse.PLoS Genet. 2006 Aug 25;2(8):e118. doi: 10.1371/journal.pgen.0020118. PLoS Genet. 2006. PMID: 16933996 Free PMC article.
-
Large-scale mutational analysis for the annotation of the mouse genome.Curr Opin Chem Biol. 2002 Feb;6(1):17-23. doi: 10.1016/s1367-5931(01)00277-0. Curr Opin Chem Biol. 2002. PMID: 11827818 Review.
-
The mouse genome.Genome Dyn. 2006;2:33-45. doi: 10.1159/000095091. Genome Dyn. 2006. PMID: 18753767 Review.
-
Mouse phenogenomics, toolbox for functional annotation of human genome.BMB Rep. 2010 Feb;43(2):79-90. doi: 10.5483/bmbrep.2010.43.2.079. BMB Rep. 2010. PMID: 20193125 Review.
-
An Experimental Approach to Genome Annotation: This report is based on a colloquium sponsored by the American Academy of Microbiology held July 19-20, 2004, in Washington, DC.Washington (DC): American Society for Microbiology; 2004. Washington (DC): American Society for Microbiology; 2004. PMID: 33001599 Free Books & Documents. Review.
Cited by
-
Lessons from Animal Models in Sjögren's Syndrome.Int J Mol Sci. 2023 Aug 20;24(16):12995. doi: 10.3390/ijms241612995. Int J Mol Sci. 2023. PMID: 37629175 Free PMC article. Review.
-
SCSA: A Cell Type Annotation Tool for Single-Cell RNA-seq Data.Front Genet. 2020 May 12;11:490. doi: 10.3389/fgene.2020.00490. eCollection 2020. Front Genet. 2020. PMID: 32477414 Free PMC article.
-
An atlas of evidence-based phenotypic associations across the mouse phenome.Sci Rep. 2020 Mar 3;10(1):3957. doi: 10.1038/s41598-020-60891-w. Sci Rep. 2020. PMID: 32127602 Free PMC article.
-
A large-scale resource for tissue-specific CRISPR mutagenesis in Drosophila.Elife. 2020 Feb 13;9:e53865. doi: 10.7554/eLife.53865. Elife. 2020. PMID: 32053108 Free PMC article.
-
Phenotyping Thermal Responses of Yeasts and Yeast-like Microorganisms at the Individual and Population Levels: Proof-of-Concept, Development and Application of an Experimental Framework to a Plant Pathogen.Microb Ecol. 2019 Jul;78(1):42-56. doi: 10.1007/s00248-018-1253-6. Epub 2018 Oct 2. Microb Ecol. 2019. PMID: 30280234
Publication types
MeSH terms
Grants and funding
LinkOut - more resources
Full Text Sources
